Interference of Recreational Intake of 1, 3, 7-Trimethylxanthine on Sensorimotor, Cognitive Functions and Fasting Blood Glucose in Wistar Rats

Bright Chisozum Robinson

Department of Human Physiology, College of Health Sciences University of Port Harcourt, Port Harcourt, Choba, Rivers State, Nigeria.

Arthur Nwafor Chuemere

Department of Human Physiology, College of Health Sciences University of Port Harcourt, Port Harcourt, Choba, Rivers State, Nigeria.

Bruno Chukwuemeka Chinko

Department of Human Physiology, College of Health Sciences University of Port Harcourt, Port Harcourt, Choba, Rivers State, Nigeria.

Blessing Ukoro *

Department of Human Physiology, College of Health Sciences University of Port Harcourt, Port Harcourt, Choba, Rivers State, Nigeria.

*Author to whom correspondence should be addressed.


1, 3, 7-Trimethylxanthine (Caffeine) is a natural alkaloid found in coffee beans, tea leaves, cocoa beans, cola nut etc. It is probably the most frequently ingested pharmacologically active substance in the world at large. This study was carried out to evaluate the effects of recreational intake of caffeine (methyl-xanthine) on sensorimotor and cognitomotor functions and fasting blood glucose in rat model. In this study, a total of twenty (20) wistar rats were randomly divided into 4 groups. After three (3) weeks of acclimatization, caffeine was administered to the rats as follows; Group1 (control) received normal water, group 2 was treated with 0.4g/ml of caffeine, group3 – 0.8g/ml of caffeine, and group4 – 1.6g/ml of caffeine, all for a period of thirty (30) days. Opaque maze (memory), elevated maze (intelligent and anxiety), beam walking (learning) and swimming/climbing test (learning), were used to evaluate sensory, motor, and cognitive performances of the rats in both control group and treated groups. Also glucometer with fine test strips was used to determine the blood glucose level of the rats at day 1, day 5, day 10, 21 and 30 during the period of caffeine administration. Data was obtained and inferentially analysed using ANOVA (SPSS version 23). The result from this study showed that caffeine significantly (p < 0.05) interfered negatively with cognitive performance in the group treated with 1.6g/ml of caffeine and also significantly decreased blood glucose level in low dose treated group and increase blood glucose at higher doses. In conclusion, Caffeine was found to improve sustained cognitive functions at medium doses (0.8g/ml), sustained motor steadiness at low (0.4g/ml) and moderate doses (0.8g/ml) but at higher doses produced uncoordinated movement.

Keywords: Caffeine, sensorimotor, cognition, learning and memory, blood glucose

How to Cite

Robinson , B. C., Chuemere , A. N., Chinko , B. C., & Ukoro , B. (2023). Interference of Recreational Intake of 1, 3, 7-Trimethylxanthine on Sensorimotor, Cognitive Functions and Fasting Blood Glucose in Wistar Rats. Asian Journal of Research and Reports in Neurology, 6(1), 103–110. Retrieved from


Barone JJ, Roberts HR. Caffeine consumption. Food Chem Toxicol. 1996;34:119–26.

Andrews KW, Schweitzer A, Zhao C, Holden JM, Roseland JM, Brandt M, Dwyer JT, Picciano MF, Saldanha LG, Fisher KD, Yetley E, Betz JM, Douglass L. The caffeine contents of dietary supplements commonly purchased in the US: analysis of 53 products with caffeine-containing ingredients. Analytical and Bioanalytical Chemistry. 2007;389: 231–9.

Gilbert RM. Caffeine consumption. In G. A. Spiller (Ed.), The methylxanthine beverages and foods: Chemistry, consumption, and health effects. New York: Liss. 1984;185-213.

Ogawa N, Ueki H. Clinical importance of caffeine dependence and abuse. Psychiatry and Clinical Neurosciences. 2007;61(3):263-268.‏

McCusker RR, Goldberger BA, Cone EJ. Caffeine content of specialty coffees. Journal of Analytical Toxicology. 2003; 27(7):520-522.‏

Heck CI, De Mejia EG. Yerba mate tea (Ilex paraguariensis): a comprehensive review on chemistry, health implications, and technological considerations. Journal of Food Science. 2007;72:138–51.

Channel Check. 2008. Bev Spect 6: 14–7.

Nawrot P, Jordan S, Eastwood J, Rotstein J, Hugenholtz A, Feely M. Effects of caffeine on human health. Food Additives and Contaminants. 2003;20:1–30.

Bonati M, Latini R, Galletti F, Young JF, Tognoni G, Garattini S. Caffeine disposition after oral doses. Clinical Pharmacology & Therapeutics. 1982;32 :98–106.

Kaplan GB, Greenblatt DJ, Ehrenberg BL, Goddard JE, Cotreau MM, Harmatz JS, Shader RI. Dose dependent pharmacokinetics and psychomotor effects of caffeine in humans. Journal of Clinical Pharmacology. 1997;37:693–703.

Chvasta TE, Cooke AR. Absorption and emptying of caffeine from the human stomach. Gastroenterology. 1971;61:838–43.

Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27:1047–53.

Higdon JV, Frei B. Coffee and health: a review of recent human research. Crit Rev Food Science and Nutrition. 2006;46:101–23.

Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. Journal of Neuroscience Methods. 1984;11:47–60.

Hennessy, Michael & Voith, Victoria & Mazzei, Samuel & Buttram, Jeff & Miller, Deborah & Linden, Fran. Behavior and cortisol levels of dogs in a public animal shelter, and an exploration of the ability of these measures to predict problem behavior after adoption. Applied Animal Behaviour Science. 2001;73. 217-233.


Southwell AL, Ko J, Patterson PH. Intrabody gene therapy ameliorates motor, cognitive, and neuropathological symptoms in multiple mouse models of Huntington's disease. Journal-of-Neuroscience. 2009;29:13589–13602.

Carter RJ, Morton J, Dunnett SB. Motor coordination and balance in rodents. Curr Protoc Neurosci. 2001;Chapter 8.

Winston AP, Hardwick E, Jaberi N. "Neuropsychiatric effects of caffeine". Advances in Psychiatric Treatment. 2005; 11(6):432–439.


Retrieved 19 December 2013

Fiani B, Zhu L, Musch BL, Briceno S, Andel R, Sadeq N, Ansari AZ. The Neurophysiology of Caffeine as a Central Nervous System Stimulant and the Resultant Effects on Cognitive Function. Cureus. 2021;13(5):e15032.


Wang GJ, Volkow ND, Logan J, Alexoff D, Fowler JS, Thanos PK, Wong C, Casado V, Ferre S, Tomasi D. Caffeine increases striatal dopamine D 2/D 3 receptor availability in the human brain. Translational Psychiatry; 2015.

Lara DR. Caffeine, mental health, and psychiatric disorders. ournal of Alzheimer's Disease. 2010;20:S239–S248.

Haskell CF, Kennedy DO, Wesnes Keith A, Scholey AB. Cognitive and mood improvements of caffeine in habitual consumers and habitual non-consumers of caffeine. Psychopharmacology. 2005;179: 813–825.

Van Gelder, van Gelder BM, Buijsse B, Tijhuis M, Kalmijn S, Giampaoli S, Nissinen A, et al. Coffee consumption is inversely associated with cognitive decline in elderly European men: the FINE Study Coffee consumption and cognitive decline European Journal of Clinical Nutrition. 2007;61:226-232

Ferré S. An update in the mechanisms of the psychostimulant effects of caffeine. Journal of Neurochemistry. 2008; 105:1067–1079.

Kaplan GB, Greenblatt DJ, Ehrenberg BL, et al. Dose-dependent pharmacokinetics and psychomotor effects of caffeine in humans. Clinical Pharmacology. 1997; 37:693–703.

Lorist M, Tops MM. Caffeine, fatigue and cognition. Brain Cogn. 2003;53: 82–94.

Doherty M, Smith PM. Effects of caffeine ingestion on exercise testing: A meta-analysis. International Journal of Sport Nutrition and Exercise Metabolism. 2004;14:626–646.

Riksen NP, Rongen GA, Smits P. Acute and long-term cardiovascular effects of coffee: Implications for coronary heart disease. Pharmacology & Therapeutics. 2009;121:185–191.

Smit HJ, Rogers PJ. Effects of energy drinks on mood and mental performance: Critical methodology. Food Quality & Prefer. 2002;13:317–326.

Cysneiros RM, Farkas D, Harmatz JS, et al. Pharmacokinetics and pharmacodynamic interactions between zolpidem and caffeine. Clinical Pharmacology & Therapeutics. 2007;82: 54–62

Shi X, Xue W, Liang S, Zhao J, Zhang X. Acute caffeine ingestion reduces insulin sensitivity in healthy subjects: a systematic review and meta-analysis. Nutrition Journal. 2016;15(1):103.


Loureiro LMR, Reis CEG, Da Costa THM. Effects of coffee components on muscle glycogen recovery: A systematic review. Int. J. Sport Nutr. Exerc. Metab. 2018; 28:284–293.

Lawler TP, Cialdella KL. Non-carbohydrate Dietary Factors and Their Influence on Post-Exercise Glycogen Storage: A Review. Current Nutrition Reports. 2020; 9:394–404.

Lane JD, Barkauskas CE, Surwit RS, Feinglos MN: 2004) Caffeine impairs glucose metabolism in type 2 diabetes (Brief Report). Diabetes (Care 27 2047–2048).