Lipid Peroxidation and Glutathione Level Following Scopolamine-Induced Cognitive Dysfunction in Rats: Potentials of Telfairia occidentalis Seeds and Talinium triangulare Leaves Aqueous Extracts
Published: 2024-08-30
Page: 98-106
Issue: 2024 - Volume 7 [Issue 1]
Samson O. Paulinus
Department of Radiography & Radiological Science, University of Calabar, Calabar, Nigeria.
Eru M. Eru *
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Gabriel U. Udo-Affah
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Michael E. Oku
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Ifiok Bassey
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Kelechi C. Uruakpa
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Nsikak M. Umoh
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Michael Ajen
Department of Biochemistry, University of Calabar, Calabar, Nigeria.
Sadeyeng E. Anani
Department of Anatomy, University of Calabar, Calabar, Nigeria.
Anozeng O. Igiri
Department of Anatomy, University of Calabar, Calabar, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: Oxidative stress augmented with progressive age, causes changes in mitochondrial DNA, mitochondrial disruption and more oxidative trouble. This process is facilitated in Alzheimer’s disease by the pathology of αβ amyloid and activated microglia.
Objectives: The present study compared the potentials of aqueous extract of Telfairia occidentalis (TO) seeds and Talinum triangulare (TT) on the malondialdehyde (MDA) and glutathione (GSH) levels following scopolamine hydrobromide (SHB)-induced Alzheimer’s type cognitive dysfunction in rats.
Methods: Forty-two Wistar rats were arrayed into seven arrays (I-VII). Alzheimer’s type cognitive dysfunction was induced in arrays II-VII by administering intraperitoneally (IP) 1 mg/kg body weight (BW) of SHB for seven days before aqueous extracts of TO (850 mg/kg and 1750 mg/kg), TT (850 mg/kg and 1750 mg/kg) and donepezil (1 mg/kg) administrations for 14 days. The rats’ blood serums were taken and analyzed.
Result: The MDA estimation in group II (672±39.65), VI (707.67±24.99) and VII (671.64±32.07) increased significantly compared with others (P<0.05). Glutathione level was significantly increased in arrays I (0.44±0.05), IV (0.41±0.08), V (0.46±0.07) and VII (0.44±0.05) compared to others.
Conclusion: Aqueous extract of TO seeds reduced MDA levels and both extracts increased glutathione levels in Alzheimer’s type cognitive dysfunction rats; though the effect of TT was dose-dependent.
Keywords: Alzheimer’s disease, glutathione, lipid peroxidase, oxidative stress, wistar rats